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KMID : 0353320130370020073
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Oral Biology Research
2013 Volume.37 No. 2 p.73 ~ p.81
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Influence of Carthamus tinctorius seed extract on proliferation and differentiation of MC3T3-E1 osteoblast cells
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Lee Sung-Yong
Kim Su-Gwan
Lee Seul-Ah
Park Min-Gyeong
Park Sun-Young
Oh Ji-Su
Kim Jin-Soo
Lee Dong-Seol
Moon So-Ra
Mo Shin-Yeob
Cho Seon-Ho
Choi Mi-Suk
Kim Chun-Sung
Cho Kwang-Hee
Kim Do-Kyung
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Abstract
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Purpose: The main aim of this study was to determine the effect of Carthamus tinctorius seed extract in the proliferation and differentiation of MC3T3-E1 osteoblastic cell line.
Materials and Methods: To determine the characteristic alteration of MC3T3-E1 cells by C. tinctorius, it was examined by cell culture, MTT assay, Von Kossa staining, reverse transcriptase-polymerase chain reaction and quantitative real-time polymerase chain reaction analysis in MC3T3-E1 cells.
Results: The treatment of C. tinctorius did not significantly affect the cell proliferation in MC3T3-E1 cells. C. tinctorius significantly accelerated the mineralization as compared with the control culture in the differentiation of MC3T3-E1 cells. C. tinctorius significantly increased the expression of alkaline phosphatase mRNA as compared with the control in the differentiation of MC3T3-E1 cells. C. tinctorius significantly accelerated the decrease of osteonectin mRNA expression as compared with the control in a time-dependent manner in the differentiation of MC3T3-E1 cells. Carthamus tinctorius significantly increased the expression of osteocalcin mRNA as compared with the control in the differentiation of MC3T3-E1 cells.
Conclusion: These results suggest that the C. tinctorius facilitate the osteoblast differentiation and mineralization in the differentiation of MC3T3-E1 osteoblastic cells, and therefore, that it may have potential properties for development and its clinical application of bone regeneration materials.
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KEYWORD
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Bone regeneration, Carthamus tinctorius, Differentiation, Mineralization, Osteoblasts
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